principles of navigation by joseph and rewari pdf

75% of PLWHA and 85% of HIVneg had detectable SARS-CoV-2-specific antibodies, with IgG levels not differing between groups. Mean time from symptoms onset to donation was 44 days (IQR: 29.5 to 55) for HIVneg and 62 (IQR: 35 to 93) for PLWHA. All individuals presented mild/moderate COVID-19. HIVneg median age was 41 (IQR: 35 to 57). The frequency and phenotype of bulk T, B and NK cells were assessed by flow cytometry. IFN-g-secreting cells were detected by ELISPOT using SARS-CoV-2 Spike, RBD or Nucleocapsid protein (10 mg/mL,) or overlapping peptide pools spanning Spike or Nucleocapsid proteins (1mg/mL). Antibody neutralization capacity was evaluated against wild-type SARS-CoV-2. SARS-CoV-2-specific IgG/IgM levels and IgG titres were determined by ELISA (COVIDAR test). Methods: Samples from donors to the Argentinean Biobank of Infectious Diseases with COVID-19 diagnosis: Twenty-one PLWHA on ART and 21 HIV negative (HIVneg) were included. The impact of HIV over the immune profile of lymphocyte populations in PLWHA recovered from COVID-19, as well as the humoral and cellular response secondary to COVID-19 were evaluated. 4Universidad de Buenos Aires, Facultad de Medicina, Departamento de Microbiología, Parasitología e Inmunología, Buenos Aires, Argentinaīackground: SARS-CoV-2-specific immune response features in PLWHA remain to be fully elucidated. 3BBEI Working Group, Buenos Aires, Argentina.

2Universidad de Buenos Aires, Facultad de Medicina, Buenos Aires, Argentina. ML Polo 1,2 A Czernikier 1,2 D Gianone 1,2 MB Vecchione 2 Y Ghiglione 1,2 S Balinotti 3 G Turk 1,4 Y Longueira 1,2 N Laufer 1,4 F Quiroga 1,4 and BBEI working groupġCONICET, Instituto INBIRS, Buenos Aires, Argentina. OALA0101 SARS-CoV-2 immunity in COVID-19 convalescent individuals living with HIV: bulk immune profiling and SARS-CoV-2-specific humoral and cellular immune responses

Principles of navigation by joseph and rewari pdf